Publications

Busnena B., Beuerle T., Mahnkopp-Dirks F., Winkelmann T., Beerhues L., Liu B. (2021.0). Formation and exudation of biphenyl and dibenzofuran phytoalexins by roots of the apple rootstock M26 grown in apple replant disease soil. Phytochemistry, 192(), 112972. doi:10.1016/j.phytochem.2021.112972

Apple replant disease (ARD) is a severe soil-borne disease frequently observed in apple tree nurseries and orchards worldwide. One of the responses of apple trees to ARD is the formation of biphenyl and dibenzofuran phytoalexins in their roots. However, there is no information on whether or not thes…

Rohr A., Staudt J., Cziborra K., Fritz A., Schmitz M., Winkelmann T. (2021.0). Split-root approach reveals localized root responses towards apple replant disease (ARD) in terms of ARD biomarker gene expression and content of phenolic compounds. Scientia Horticulturae, 286(), 110117. doi:10.1016/j.scienta.2021.110117

Apple replant disease (ARD) is a threat to apple fruit and tree production worldwide caused by shifts in the soil microbiome. Chemical and physical measures of soil disinfection are the most employed countermeasures but inter-row plantings demonstrate that ARD is widely immobile in the soil, which c…

Benning S., Brugnone N., Siani R., Kublik S., Schloter M., Rad V. (2021.0). Complete Genome Sequences of Two Rhodococcus sp. Strains with Large and Linear Chromosomes, Isolated from Apple Rhizosphere. Microbiology Resource Announcements, 10(24), . doi:10.1128/mra.00159-21

Members of the genus Rhodococcus are usually able to catalyze a number of processes, which are of great interest for ecosystem performance as well as biotechnology. Here, we report the complete genome sequences of two Rhodococcus strains that were isolated from rhizosphere soil from an apple orchard…

Armin R., Zühlke S., Grunewaldt-Stöcker G., Mahnkopp-Dirks F., Kusari S. (2021.0). Production of Siderophores by an Apple Root-Associated Streptomyces ciscaucasicus Strain GS2 Using Chemical and Biological OSMAC Approaches. Molecules, 26(12), 3517. doi:10.3390/molecules26123517

Apple Replant Disease (ARD) is a significant problem in apple orchards that causes root tissue damage, stunted plant growth, and decline in fruit quality, size, and overall yield. Dysbiosis of apple root-associated microbiome and selective richness of Streptomyces species in the rhizosphere typicall…

Armin R., Zühlke S., Mahnkopp-Dirks F., Winkelmann T., Kusari S. (2021.0). Evaluation of Apple Root-Associated Endophytic Streptomyces pulveraceus Strain ES16 by an OSMAC-Assisted Metabolomics Approach. Frontiers in Sustainable Food Systems, 5(), . doi:10.3389/fsufs.2021.643225

The One Strain Many Compounds approach (OSMAC) is a powerful and comprehensive method that enables the chemo-diversity evaluation of microorganisms. This is achieved by variations of physicochemical cultivation parameters and by providing biotic and abiotic triggers to mimic microorganisms' natural …

Reim S., Cestaro A., Siewert C., Wöhner T., Mahnkopp-Dirks F., Winkelmann T., Hanke M., Flachowsky H. (2021.0). Evaluation of tolerance to apple replant disease (ARD) inMalusgermplasm. Acta Horticulturae, (1307), 327-334. doi:10.17660/actahortic.2021.1307.50

Apple replant disease (ARD), also described as soil decline, is known for centuries; however, up to now the ultimate causes are unknown. After replanting plants of the same species, the plants are characterized by a poor vegetative development, stunted growth and reduced yield in terms of quantity a…

Grunewaldt-Stöcker, G., Popp, C., Wamhoff, D., Maiss, E. and Winkelmann, T. (2021.0). Microscopic evidence of Nectriaceae and other microbes in infected fine root tissue of replant diseased apple plants. European Journal of Horticultural Science, (), . doi:doi.org/10.17660/eJHS.2021/86.1.4

Fine roots of apple rootstocks ‘M26’ and ‘Bittenfelder Sämling’ showing symptoms of apple replant disease (ARD) were histologically examined to clarify the relationships of microbial endophytes to tissue and cell damages. The plants were either grown on ARD-infested fields at three different locatio…

Balbín-Suárez A., Jacquiod S., Rohr A., Liu B., Flachowsky H., Winkelmann T., Beerhues L., Nesme J., Sørensen S., Vetterlein D., Smalla K. (2021.0). Root exposure to apple replant disease soil triggers local defense response and rhizoplane microbiome dysbiosis. FEMS Microbiology Ecology, (), . doi:10.1093/femsec/fiab031

A soil column split-root experiment was designed to investigate the ability of apple replant disease (ARD) causing agents to spread in soil. ‘M26’ apple rootstocks grew into a top layer of Control soil, followed by a barrier-free split-soil layer (Control soil/ARD soil). We observed a severely reduc…

Flachowsky, H., Winkelmann, T. (2021.0). Bodenmüdigkeit im Fokus: Die Reaktionen von Apfelpflanzen verstehen, um tolerante Unterlagen zu identifizieren. Obstbau, 46(5), S. 267-272.